1.3.1 ( Lowe and Eddy, 1997), respectively, were used. To identify ribosomal RNA and transfer RNA, RNAmmer 1.2 ( Lagesen et al., 2007) and tRNAscan v. A total of 6,939,438 reads (with 688-fold coverage) were assembled using CLC Genomics Workbench 7.5.1 with default parameters as follows: masking mode, no masking mismatch cost, 2 insertion cost, 3 deletion cost, 3 length cost, 3 length fraction, 0.5 similarity fraction, 0.8 global alignment, no auto-detect paired distances, yes non-specific match handling map, randomly. thermophila CBA1501 T were sequenced at a read length of 300 bp using the Illumina MiSeq system, with paired-end library constructed using the Nextera DNA Library Prep kit (illumina, USA), according to the manufacturer's instructions ( Moon et al., 2015). Genome Sequencing, Assembly, and Annotation Its genomic DNA was extracted using the G-spin total DNA extraction kit (iNtRON Biotechnology, Korea) and QuickGene DNA tissue kit S (Kurabo, Japan). For DNA extraction, the strain was enriched at 80☌ in M236 medium, using a serum bottle. 236 (M236) (containing per liter salt base solution: 2.94 g trisodium citrate dihydrate, 0.5 g yeast extract, 10.0 ml trace vitamins, 1.0 mg resazurin, 0.5 g Na 2S♹H 2O, and 20 mM thiosulfate). thermophila CBA1501 T from the solfataric soil of the Mayon volcano in the Republic of the Philippines ( Yim et al., 2015) and cultivated it on modified JCM medium no. Materials and Methods Culture Conditions and DNA Extraction thermophila CBA1501 T has been reported and information of hyperthermophilic enzymes of high biotechnological value has been provided. thermophila CBA1501 T (= ATCC BAA-2415 T = JCM 17228 T) was isolated from solfataric soil in the Republic of the Philippines ( Yim et al., 2015). These enzymes can be studied using model systems to elucidate enzyme mechanisms and evolution of proteins stable at high temperatures and to determine the higher temperature limit for enzyme stability ( Vieille and Zeikus, 2001). Hyperthermophilic enzymes are stable and active at high temperatures of >70☌ ( Vieille et al., 1996). distributa and “ Vulcanisaeta moutnovskia” ( Mavromatis et al., 2010 Gumerov et al., 2011), have been reported for the genus Vulcanisaeta, as per the NCBI genome database ( ). To date, 15 genomes, including two complete genomes, V. Members of the genus Vulcanisaeta are rod-shaped, anaerobic, hyperthermophilic, and acidophilic ( Itoh et al., 2002). thermophila ( Yim et al., 2015), as per the List of Prokaryotic Names with Standing in Nomenclature database ( Parte, 2014). It currently includes 3 validly named species, that is, Vulcanisaeta distributa ( Itoh et al., 2002), V. The genus Vulcanisaeta belongs to the family Thermoproteaceae, order Thermoproteales, phylum Crenarchaeota, and was first proposed by Itoh et al. #0 Foam::error::printStack(Foam::Ostream&) at ?:? #1 Foam::error::abort() at ?:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy>::calculate() at ?:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy>::correct() at ?:? #4 ? at ?:? #5 _libc_start_main in "/lib/x86_64-linux-gnu/libc.so.Hyperthermophilic archaea have been isolated from high-temperature environments such as geothermally heated soils, sulfur-rich hot springs, and submarine volcanic habitats optimal growth of these organisms occurs above 80☌ ( Stetter, 1999, 2006, 2013). > FOAM FATAL ERROR: Maximum number of iterations exceededįrom function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. I have some problem with chtMultiRegionSimpleFoam
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